Protocols

Plastic pan traps with soapy water deployed along stream transects to capture terrestrial invertebrates falling into or near the river. Traps are pooled by transect and processed for taxonomic identif...

Collection of nitrogen samples via syringe filtration and chlorophyll samples via algae collection from submerged rocks, with spectrophotometric analysis normalized by substrate area.

Collection and processing of biofilm samples from natural and artificial substrates to quantify biomass, algal content, and nutrient content through AFDM, chlorophyll-a, and phosphorus analysis.

Three-pass electrofishing to estimate fish densities and species composition in stream reaches for characterizing predator presence and abundance.

Method for collecting fish skin mucus by gentle rubbing in plastic bags followed by freeze-drying and chemical characterization of mucus components including glycosaminoglycans, hexosamine, and uronic...

Standardized substrate tiles deployed in stream channels for algae colonization, followed by systematic scraping, filtering, and microscopic enumeration of didymo cells.