Fish mucus extraction and processing (Baetidae)

Subcategory: chemical extraction

Standard reference: Landeira-Dabarca, Sieiro, & Alvarez, 2013

Papers: 2 | Mentions: 2

Local Knowledge Graph (15 entities)

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Description

Method for collecting fish skin mucus by gentle rubbing in plastic bags followed by freeze-drying and chemical characterization of mucus components including glycosaminoglycans, hexosamine, and uronic acid.

Typical Equipment

cotton
plastic bags
micropipette
preweighed vials
speed-vacuum freeze dryer

Output Measurements

mucus dry weight
water content
sulfated sugar concentration
hexosamine concentration
uronic acid concentration

Papers Using This Protocol (2)

article

Mayflies avoid sweets: fish skin mucus amino sugars stimulate predator avoidance behaviour of <i> Baetis </i> larvae

2019Animal Behaviourusing

article

Origin and specificity of predatory fish cues detected by Baetis larvae (<i>Ephemeroptera; Insecta</i>)

2014Animal Behaviourusing