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Protocols

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2 protocols

laboratorystandardized

CRISPR-Cas9 gene editing (Pieridae)

DNA extraction, tagmentation-based library preparation, and Illumina sequencing to generate whole-genome sequence data from butterfly specimens. Modified DNeasy protocol followed by Tn5 tagmentation a...

genomic sequencing4 papersDNeasy Blood & Tissue Kit, NanoDrop 2000/2000c...
laboratorystandardized

RNA-seq (Drosophilidae)

Illumina GAIIx RNA sequencing of larval samples from different plant treatments followed by differential expression analysis to identify genes responsive to plant chemical defenses.

transcriptomics2 papersQiagen Tissue Lysis Buffer, gDNA Eliminator spin column...