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CRISPR-Cas9 gene editing (Pieridae)

Subcategory: genomic sequencing
Standard reference: Andolfatto et al. (2011) for library preparation protocol
Papers: 4 | Mentions: 4

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Description

DNA extraction, tagmentation-based library preparation, and Illumina sequencing to generate whole-genome sequence data from butterfly specimens. Modified DNeasy protocol followed by Tn5 tagmentation and PCR amplification.

Typical Equipment

  • DNeasy Blood & Tissue Kit
  • NanoDrop 2000/2000c
  • Tn5 transposase
  • Start Taq DNA polymerase
  • thermal cycler
  • AMPureXP beads
  • Illumina NovaSeq S4

Output Measurements

  • whole-genome sequence reads
  • SNP genotypes

Papers Using This Protocol (4)

article

Genetic basis of an adaptive polymorphism controlling butterfly silver iridescence

2025Current Biologyusing
article

Patterns of genetic variation and local adaptation of a native herbivore to a lethal invasive plant

2024Molecular Ecologyusing
article

Evidence for a single, ancient origin of a genus-wide alternative life history strategy

2023Science Advancesusing
article

The genome of the Margined White butterfly (Pieris macdunnoughii): sex chromosome insights and the power of polishing with PoolSeq data

2021Genome Biology and Evolutionusing