Protocols

Wood samples are sectioned into sub-annual increments and cellulose is extracted for oxygen and carbon isotope analysis to assess seasonal water sources and water use efficiency in trees.

Meta-analytical approach to synthesize population-level climate sensitivities across multiple species using structured population models and standardized effect sizes. Incorporates life history traits...

Uses hyperspectral imaging (400-1000 nm) combined with Partial Least Squares Regression to correlate spectral signatures with measured leaf traits like LMA and LWC. Enables non-destructive remote meas...

PLSR modeling approach using leaf spectral reflectance measurements to extrapolate functional trait values from subset of direct measurements to larger sample sizes. Ten-fold cross-validation used for...

Processing of gridded climate data from the CRU TS3.1 dataset with lapse rate adjustment for elevation differences between grid cells and actual study locations, validated against local meteorological...

Laboratory analysis of plant samples for nitrogen content using elemental CHNSO analyzer and water content determination through oven-drying weight loss.

Photographic analysis using ImageJ software with ellipsis tool to quantify percent vegetation coverage around each sampling point as a fraction of total photographed area.

Systematic compilation and cleaning of plant trait records from multiple databases to create a comprehensive tundra plant trait dataset. Combined TRY database records with Tundra Trait Team contributi...

Compilation and analysis of published plant population divergence data paired with evolvability estimates to test macroevolutionary predictions. Uses proportional divergence metrics and meta-analytica...

Laboratory technique to determine ploidy level, validated against Hordeum vulgare standards.

Collection of site spectra to validate airborne hyperspectral data acquired by NEON Aerial Observation Platform.

Genotyping using a panel of 384 candidate-gene single nucleotide polymorphisms putatively associated with adaptive traits.