Protocols

Quantitative analysis of pollen grain composition in bee provisions using compound microscopy. Pollen samples stained with fuchsin and examined at 100× magnification with systematic transect counting ...

Using Generalized Additive Models to estimate emergence, peak, and senescence timing from sparse time-series abundance data. Models fit abundance curves and extract phenological metrics from first and...

Analysis of larval fecal pellets to assess digestive efficiency by measuring frass mass relative to provision mass and quantifying undigested pollen grains using microscopy and staining techniques.

Bayesian mixed effects modeling of body size distributions using four statistical moments (mean, variance, skew, kurtosis) to characterize trait filtering processes. Uses robust measures and hierarchi...

Statistical approach to identify correlations between species traits and environmental variables by calculating Pearson correlations between climate variables and trait values across species, weighted...

Systematic search and evaluation of published literature examining relationships between phenological shifts and demographic consequences using specific search criteria in Web of Science.

Statistical method to compare species richness across samples with different sampling efforts by generating rarefaction curves. Uses individual-based approach to generate rate of visitor richness accu...

Compilation and processing of NOAA weather data for degree-day calculations from 1950-2015. Temperature data aggregation for thermal ecology analysis.