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Controlled frost sensitivity chamber experiment (Plantae)
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Description
Plant tissues are subjected to controlled freezing temperatures in a programmable chamber to determine frost damage thresholds. Temperatures are gradually reduced to simulate natural frost events, then damage is assessed visually.
Typical Equipment
- Sigma M26-C4 programmable temperature chamber
- liquid CO₂
- aluminum foil
Output Measurements
- frost damage binary scores
- LT₁₀, LT₅₀, LT₉₀ temperature thresholds
Papers Using This Protocol (3)
article
Frost sensitivity of leaves and flowers of subalpine plants is related to tissue type and phenology.
student_paper
Frost Sensitivity of Subalpine Plants in the Colorado Rocky Mountains: The Effects of Seasonality, Water Content, and Phylogeny
student_paper