← Back to Protocolsexperimental
Stable isotope enrichment marking of aquatic insects (Animalia)
Local Knowledge Graph (10 entities)
Loading graph...
Description
A technique to mark developing aquatic insect larvae by enriching their habitat with heavy nitrogen isotopes (15N), which becomes incorporated into larval tissues and persists through metamorphosis to adults. Adults can then be identified as originating from the enriched habitat based on their isotopic signature.
Typical Equipment
- 10 hand pump bug sprayer
- Isotech Inc. 15N enriched ammonium chloride
- PDZ Europa Hydra 20-20 mass spectrometer
- PDZ Europa ANCA-GSL elemental analyzer
Output Measurements
- δ15N isotopic composition
- marked vs unmarked classification