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Bumble bee pathogen microscopy survey (Apidae)

Subcategory: parasite detection
Papers: 1 | Mentions: 1

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Description

Dissection of bumble bees followed by microscopic examination of gut and fat body tissues for protozoan parasites using Giemsa staining. Quantifies pathogen intensity by counting cells in standardized fields of view.

Typical Equipment

  • dissection tools
  • microscope slides
  • methanol
  • Giemsa solution
  • phosphate buffer
  • water
  • compound microscope

Output Measurements

  • pathogen presence/absence
  • pathogen intensity (cell counts)
  • infection prevalence