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Bumble bee pathogen microscopy survey (Apidae)
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Description
Dissection of bumble bees followed by microscopic examination of gut and fat body tissues for protozoan parasites using Giemsa staining. Quantifies pathogen intensity by counting cells in standardized fields of view.
Typical Equipment
- dissection tools
- microscope slides
- methanol
- Giemsa solution
- phosphate buffer
- water
- compound microscope
Output Measurements
- pathogen presence/absence
- pathogen intensity (cell counts)
- infection prevalence
