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Bacterial endophyte isolation from crucifer leaves (Brassicaceae)
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Description
Surface sterilization of plant leaves followed by tissue plating on selective media to isolate bacterial endophytes and assess their growth characteristics and pathogenicity.
Typical Equipment
- cork borer
- Kings B media agar
- 70% ethanol
- blunt syringe
- solid agar plates
Output Measurements
- bacterial colony counts
- growth rate
- virulence symptoms