Acute restraint stress protocol (Ambystomatidae)

Subcategory: stress induction

Standard reference: Romero and Reed, 2005

Papers: 1 | Mentions: 1

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Description

Standardized protocol for inducing acute stress in amphibians through manual restraint to measure stress hormone response over time. Includes baseline sampling followed by stress induction and temporal monitoring of stress hormone levels.

Typical Equipment

dip nets
plastic buckets
cotton-tipped wooden swab precut to 4 cm

Output Measurements

stress hormone response curves
baseline corticosterone
stress-induced corticosterone

Papers Using This Protocol (1)

student_paper

Morph- and sex-specific differences in corticosterone of Arizona tiger salamanders (<i>Ambystoma mavortium nebulosum</i>)

2020using