Lepidopteran fat body whole-mount immunofluorescence (Nymphalidae)

Subcategory: immunostaining

Papers: 1 | Mentions: 1

Local Knowledge Graph (10 entities)

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Description

A whole-mount immunostaining protocol optimized for visualizing FOXO and mTOR protein localization in butterfly fat body tissue using species-specific antibodies. The protocol involves tissue fixation, permeabilization with detergents, blocking, and fluorescent antibody labeling.

Typical Equipment

4% paraformaldehyde
Triton X-100
Tween 20
BSA
Rabbit anti-FOXO antibody
Rabbit anti-TOR antibody
Goat anti-rabbit AlexaFluor 488
VectaShield with DAPI
nutator

Output Measurements

protein subcellular localization
immunofluorescence intensity
tissue-specific protein distribution

Papers Using This Protocol (1)

article

Antibody development to identify components of IIS and mTOR signaling pathways in lepidopteran species, a set of non-model insects

2023microPublication Biologyintroducing