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Bumble bee honey ethanol quantification (Apidae)
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Description
A laboratory protocol for extracting honey samples from captive bumble bee colonies and quantifying ethanol content using enzyme-based assays. Samples are collected over time from maintained colonies to assess natural ethanol accumulation.
Typical Equipment
- 200 μL pipette
- 1.5 mL microcentrifuge tubes
- qPCR-grade water
- parafilm
- freezer at -80°C
- balance for weighing
- handheld refractometer
- enzyme-based ethanol assay kit
- 96-well plates
- plate reader
Output Measurements
- honey ethanol concentration
- total sugar concentration
- honey mass