CTAB DNA extraction (Pinaceae)

Subcategory: phylloplane microbiome

Standard reference: Carper et al. [20]

Papers: 1 | Mentions: 1

Local Knowledge Graph (12 entities)

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Description

Modified CTAB DNA extraction protocol specifically designed to isolate microbial cells from conifer needle surfaces while excluding endophytic microorganisms from internal needle tissues.

Typical Equipment

sterile razor blades
sterile bags
PBS-S buffer
vortex
sonicator
centrifuge
CTAB reagents

Output Measurements

phylloplane microbial DNA

Papers Using This Protocol (1)

article

Host species and geographic location shape microbial diversity and functional potential in the conifer needle microbiome

2025Microbiomeusing