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COI DNA barcoding (Baetidae)

Subcategory: molecular

Standard reference: Folmer et al. (1994)

Papers: 2 | Mentions: 2

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Description

DNA extraction using modified Chelex protocols followed by PCR amplification and sequencing of the mitochondrial cytochrome oxidase I gene to assess genetic variation within and among mayfly populations.

Typical Equipment

Eppendorf tubes
chelex solution
proteinase K
PCR equipment
LCO1490 primer
HCO2198 primer
Applied Biosystems 377 automated sequencer

Output Measurements

DNA sequences
haplotype frequencies
sequence divergence

Papers Using This Protocol (2)

article

Are populations of mayflies living in adjacent fish and fishless streams genetically differentiated?

2005Freshwater Biologyusing

article

Genetic structure in a montane mayfly <i>Baetis bicaudatus</i> (Ephemeroptera: Baetidae), from the Rocky Mountains, Colorado

2003Freshwater Biologyusing