Transgenic functional complementation assay (Brassicaceae)

Subcategory: functional genomics

Papers: 1 | Mentions: 1

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Description

Creation and analysis of transgenic Arabidopsis lines expressing candidate genes from study species to test gene function and allelic effects. Uses heterologous expression system to isolate gene effects.

Typical Equipment

transformation vectors
plant growth chambers
HPLC systems

Output Measurements

transgenic plant glucosinolate levels
gene expression patterns
chemical phenotypes

Papers Using This Protocol (1)

article

A gain of function polymorphism controlling complex traits and fitness in nature

2012Scienceusing