Electrospray mass spectrometry alkaloid quantification (Plantae)

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Description

Plant tissues were extracted in methanol and analyzed by electrospray mass spectrometry to identify and quantify norditerpene alkaloids. Mass spectra were recorded over 150-800 m/z range and alkaloid concentrations calculated based on relative abundance of internal standard.

Typical Equipment

• Electrospray mass spectrometer
• Wiley mill
• Retsch mixer mill MM301
• Centrifuge

Output Measurements

• alkaloid concentrations
• alkaloid identities
• mass spectra

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