Standard diatom cleaning and mounting protocol (Bacillariophyceae)

Subcategory: morphological taxonomy

Standard reference: APHA et al. 1992

Papers: 1 | Mentions: 1

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Description

Standard protocol for collecting freshwater diatoms from various substrates, cleaning with acids to remove organic matter, and mounting for microscopic examination. Includes both light microscopy and scanning electron microscopy preparation.

Typical Equipment

tablespoon
large-bore pipette with suction bulb
Lugol's (IKI) solution
sulfuric acid (H2SO4)
potassium dichromate (K2Cr2O7)
hydrogen peroxide (H2O2)
Hyrax mounting medium
microscope slides
Leica DM LB2 research microscope
Spot Insight Model 14.0 monochrome digital camera
Hitachi S4700 Type II cold field emission SEM
aluminum stubs
Pelco Model 3 Sputter Coater
gold
palladium
Spot Software version 4.5

Output Measurements

valve morphology
valve dimensions
striae density
taxonomic identification

Papers Using This Protocol (1)

article

Neidiopsis hamiltonii sp. nov., N. weilandii sp. nov., N. levanderi and N. wulffii from western North America

2014Diatom Researchusing