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Butterfly egg biochemical composition analysis (Nymphalidae)

Subcategory: biochemical
Standard reference: Giron et al. [18]
Papers: 1 | Mentions: 1

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Description

Multi-component analysis of butterfly egg composition including protein content via Bradford dye-binding method, glycogen content via modified Giron protocol, and triglyceride content using commercial reagent kits.

Typical Equipment

  • Bradford dye-binding reagents
  • Infinity Triglycerides Stable Reagent (ThermoScientific, Waltham, MA, USA)
  • drying oven

Output Measurements

  • egg protein content
  • egg glycogen content
  • egg triglyceride content
  • egg dry mass

Papers Using This Protocol (1)

article

Effects of increased flight on the energetics and life history of the butterfly <i>Speyeria mormonia</i>

2015PLoS Oneusing