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Qiagen DNeasy Blood and Tissue Kit protocol (Limnephilidae)

Subcategory: molecular
Standard reference: Qiagen DNeasy Blood and Tissue kit manufacturer's protocol
Papers: 1 | Mentions: 1

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Description

Extraction of total genomic DNA from individual caddisfly larvae using Qiagen DNeasy Blood and Tissue kit with automated QiaCube processing after manual tissue homogenization.

Typical Equipment

  • Qiagen DNeasy Blood and Tissue kit
  • ceramic mortar and pestle
  • Qiagen QiaCube extraction robot
  • NanoDrop 2000 spectrophotometer

Output Measurements

  • total genomic DNA
  • DNA concentration
  • DNA quality metrics

Papers Using This Protocol (1)

article

Identifying PCR primers to facilitate molecular phylogenetics in Caddisflies (Trichoptera)

2015Zoological Systematicsusing