Trichoptera PCR primer screening (Limnephilidae)

Local Knowledge Graph (12 entities)

Description

Systematic testing of 107 published PCR primer pairs from multiple gene regions to identify those that successfully amplify target sequences in caddisfly species. Includes PCR amplification, gel verification, and Sanger sequencing of products.

Typical Equipment

• NEB 2X Quickload MasterMix
• Top-Taq DNA Polymerase Mastermix
• BioCycle MyiCycler
• C1000 Thermal Cycler
• S1000 Thermal Cycler
• 1% agarose slab gels
• TAE buffer
• Qiagen QiAxcel capillary electrophoresis instrument

Output Measurements

• successful primer pairs
• PCR amplification success rates
• DNA sequences

Papers Using This Protocol (1)