Bee gut bacterial isolation and genome sequencing (Orbaceae)

Subcategory: microbial isolation

Standard reference: Kwong WK, Moran NA. 2016. Gut microbial communities of social bees. Nat Rev Microbiol 14:374–384

Papers: 1 | Mentions: 1

Local Knowledge Graph (13 entities)

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Description

Isolation of pure bacterial cultures from bee gut homogenates followed by whole genome sequencing using Illumina MiSeq and PacBio platforms with subsequent assembly and annotation.

Typical Equipment

10 mM MgSO4
heart infusion agar
Illumina MiSeq
PacBio RS II

Output Measurements

bacterial isolate cultures
genome sequences
phylogenetic relationships

Papers Using This Protocol (1)

article

Metabolism of toxic sugars by the bee gut symbiont <i>Gilliamella apicola</i>

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