Plant tissue genomic DNA extraction for high molecular weight DNA (Brassicaceae)

Subcategory: molecular

Standard reference: ref. 16

Papers: 1 | Mentions: 1

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Description

Extraction of high molecular weight nuclear DNA from etiolated plant seedlings with minimal organellar contamination. Uses nuclei isolation followed by DNA purification for downstream genome sequencing.

Typical Equipment

MS liquid medium
MEB buffer
commercial blender
TE buffer

Output Measurements

high molecular weight genomic DNA

Papers Using This Protocol (1)

article

Young inversion with multiple linked QTLs under selection in a hybrid zone

2017Nature Ecology and Evolutionusing