Wild medicinal plant secondary metabolite profiling via HPLC-UV (Apiaceae)

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Description

Field collection of Ligusticum porteri roots followed by methanol extraction and HPLC-UV analysis to quantify five secondary metabolites. Method adapted from previously published work by Rivero et al. (2012).

Typical Equipment

• GPS
• LI-250 A & LI-191R light meter
• Agilent 1100 HPLC
• Restek Ultra II C18 column

Output Measurements

• compound concentrations in ppm
• retention times
• peak areas

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