Pollen tube growth and counting in Corydalis flowers (Fumariaceae)

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Description

Fixed flowers were processed through staining and clearing to visualize and count pollen tubes that had grown down the style to assess fertilization success. This involved fixation in ethanol:acetic acid, staining with fuchsin and fast green, clearing in lactic acid, and microscopic examination.

Typical Equipment

• 95% ethanol
• glacial acetic acid
• 1% basic fuchsin
• 1% fast green
• 88% lactic acid
• compound microscope

Output Measurements

• pollen tube count per flower

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