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Ant chemical associative learning field assay (Formicidae)
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Description
A two-phase field protocol using training baits scented with plant chemicals followed by two-choice tests to assess whether ants form associative memories linking plant odors to carbohydrate rewards. Ants are trained on scented baits for 24 hours, then tested for preference using choice apparatus with matching vs. non-matching scents.
Typical Equipment
- bamboo sticks
- sugar water baits
- two-choice test apparatus
- fresh plant leaves
- data recording sheets
Output Measurements
- ant presence on baits
- ant species identity
- scent preference behavior