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Honeydew collection and chemical analysis

Subcategory: biochemical
Papers: 2 | Mentions: 2

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Description

Collection of aphid honeydew on aluminum foil substrates followed by LC-MS/MS analysis to quantify sugar and amino acid composition as indicators of plant quality and ant-aphid mutualism benefits.

Typical Equipment

  • aluminum foil squares
  • stereoscope
  • LC-MS/MS system
  • ImageJ

Output Measurements

  • honeydew deposition rate
  • sugar concentrations
  • amino acid concentrations

Papers Using This Protocol (2)

article

Early snowmelt reduces aphid abundance <i>Aphis asclepiadis</i> by creating water stressed host plants <i>Ligusticum porteri</i> and altering interactions with ants

2020Arthropod-Plant Interactionsintroducing
article

Elevated temperatures alter an ant aphid mutualism

2019Entomologia Experimentalis et Applicatausing