PoolSeq vs individual short-read polishing comparison (Pieridae)

Subcategory: genome polishing

Papers: 1 | Mentions: 1

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Description

Comparative analysis of genome assembly improvement using pooled vs individual Illumina short reads for polishing long-read assemblies, assessed using BUSCO completeness metrics.

Typical Equipment

Illumina HiSeq platform
Pilon (v.1.23)
SeqKit
NextGenMap

Output Measurements

BUSCO scores
assembly completeness
ortholog hit ratios

Papers Using This Protocol (1)

article

The genome of the Margined White butterfly (Pieris macdunnoughii): sex chromosome insights and the power of polishing with PoolSeq data

2021Genome Biology and Evolutionintroducing