ONT long-read genome assembly with dual assembler approach (Pieridae)

Local Knowledge Graph (23 entities)

Description

Oxford Nanopore long-read sequencing followed by assembly using both FLYE and NECAT assemblers, with iterative polishing using Racon and Medaka, then merging assemblies using Quickmerge and HaploMerger2.

Typical Equipment

• MinION platform
• GUPPY (v.3.2.10)
• Flye (v.2.7)
• NECAT (v.0.0.1)
• Racon (v.1.4.13)
• Medaka (v.1.0.3)
• Quickmerge (v.0.3)
• HaploMerger2 (v.20180603)

Output Measurements

• draft genome assembly
• contig statistics
• N50 values

Papers Using This Protocol (2)