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PCR-based complete viral genome recovery (Sciuridae)

Subcategory: molecular
Papers: 1 | Mentions: 1

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Description

Method using abutting primers designed from contigs to amplify and recover complete circular viral genomes, followed by cloning and Sanger sequencing for verification.

Typical Equipment

  • Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, USA)
  • pJET 1.2 vector
  • Escherichia coli DH5-alpha competent cells

Output Measurements

  • complete viral genome sequences

Papers Using This Protocol (1)

article

Diverse cressdnaviruses and an anellovirus identified in the fecal samples of yellow-bellied marmots

2021Virologyusing