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microsatellite DNA analysis (Formicidae)
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Description
DNA extraction from ant specimens followed by PCR amplification of microsatellite loci and relatedness estimation using maximum likelihood methods based on population allele frequencies.
Typical Equipment
- microcentrifuge tubes
- hand drill
- lysis buffer
- PCR reagents
- Techne Laboratories thermal cycler
- polyacrylamide gels
Output Measurements
- relatedness coefficients
- allele frequencies
- genetic diversity estimates
