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microsatellite parentage assignment (Sciuridae)
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Description
DNA extraction from hair samples followed by microsatellite genotyping to determine parentage relationships and construct molecular pedigrees for quantitative genetic analysis.
Typical Equipment
- QIAmp Mini Kit by Qiagen Inc
- GENEMAPPER software
- CERVUS 3.0 software
Output Measurements
- genetic relatedness matrix
- pedigree relationships
- allele frequencies
Papers Using This Protocol (19)
article
Society formation and maintenance in yellow-bellied marmots
article
The heritability of fear: decomposing sources of variation in marmot flight initiation distance
thesis
Estimating the heritability of female dispersal in yellow-bellied marmots
article
Sex-specific reproductive strategies in wild yellow-bellied marmots (<i> Marmota flaviventer </i>): senescence and genetic variance in annual reproductive success differ between the sexes
article
Heritable variation in the timing of emergence from hibernation
article
The effect of maternal glucocorticoid levels on juvenile docility in yellow-bellied marmots
article
Genetic basis of between-individual and within-individual variance of docility
student_paper
Do Social Interactions Decrease Basal Stress Levels In Breeding Female Yellow-Bellied Marmots?
student_paper
Exploring the behavioral rules influencing sociality in a facultatively social species, yellow-bellied marmots
article
Stress hormone metabolites predict overwinter survival in yellow-bellied marmots
article
Environmental, social, morphological and behavioural constraints on opportunistic multiple paternity
article
Maternal effects on anogenital distance in a wild marmot population
article
Litter sex composition affects life-history traits in yellow-bellied marmots
article
No evidence of inbreeding avoidance despite survival costs in a polygynous rodent
article
Bottlenecks and rescue effects in a fluctuating population of golden-mantled ground squirrels (<i>Spermophilus lateralis</i>)
article
Correlates and consequences of dominance in a social rodent
article
Heritability of anti-predatory traits: vigilance and locomotor performance in marmots
article
Applying the coalitionary-traits metric: sociality without cooperation in male yellow-bellied marmots
article