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Seed surface sterilization (Fabaceae)
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Description
Common garden experiment manipulating soil microbial communities through sterilization vs. inoculation to test effects on seed germination success and timing under controlled environmental conditions.
Typical Equipment
- autoclave
- Conviron MTR30 growth chamber
- Ray Leach Cone-tainer containers
Output Measurements
- germination success
- germination latency
- days to emergence
Papers Using This Protocol (2)
article
Legume germination is delayed in dry soils and in sterile soils devoid of microbial mutualists: Species-specific implications for upward range expansions
article