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16S rRNA gene sequencing (Nymphalidae)

Subcategory: molecular identification
Papers: 1 | Mentions: 1

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Description

Standard protocol to isolate, culture, and identify bacteria using genomic DNA extraction, 16S rRNA gene sequencing, and NCBI database comparison.

Typical Equipment

  • HiMedia nutrient agar
  • DNeasy Blood and Tissue Kit
  • Hi-Seq platform

Output Measurements

  • bacterial species identification

Papers Using This Protocol (1)

article

The dispersal of microbes among and within flowers by butterflies

2023Ecological Entomologyusing