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hand-pollination crossing (Orobanchaceae)
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Description
Controlled laboratory assay using prepared stigmas on growth medium to test pollen germination and tube growth across species boundaries. Stigmas are prepared from unopened buds, treated with pure pollen loads, and incubated under controlled conditions to observe tube growth.
Typical Equipment
- paintbrush
- dissection tools
- nitrogen peroxide
- pollen tube growth medium
- petri dishes
- growth chamber
- dissecting microscope
Output Measurements
- pollen tube germination success
- pollen tube growth through stigma