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Metagenome-assembled genomes from Slate River floodplain sediments near Crested Butte, CO, USA (June to October 2020)

Creators: Anna Rasmussen
Year: 2025
DOI: 10.15485/2588874
Location: OBJ1, Floodplain sediment core, near Crested Butte, CO, USACrested Butte, CO, U.S.A., Beaver dam and legacy mining activity open range. Situated on a floodplain close to the confluence of the Oh-Be-Joyful Creek and Slate River. The site is the focus for the SLAC Groundwater Quality SFA program.
Temporal extent: 2020-06-01 to 2020-10-23
Bounding box: 38.910°N to 38.910°N, -107.029°W to -107.029°W
Publisher: ESS_DIVE
Tags: ESS-DIVE File Level Metadata Reporting Format, ESS-DIVE CSV File Formatting Guidelines Reporting Format, EARTH SCIENCE > BIOSPHERE > OMICS > METAGENOME > METAGENOME ASSEMBLED GENOMES (MAGs), EARTH SCIENCE > BIOSPHERE > OMICS > OMICS METHODOLOGY > BIN/BINNING, EARTH SCIENCE > BIOSPHERE > OMICS > BUILDING BLOCKS > CONTIG, EARTH SCIENCE > BIOSPHERE > OMICS > BUILDING BLOCKS > eDNA, EARTH SCIENCE > BIOSPHERE > OMICS > MICROBIOME, EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > MICROBIAL CHANGES, EARTH SCIENCE > LAND SURFACE > SOILS > MICROFAUNA, Genetics & Evolution, Hydrology & Watersheds, Groundwater, Soil Science, Geochemistry & Isotopes, Gunnison Basin, Research Programs

Description

Microorganisms play a key role in cycling nutrients and contaminants in the terrestrial environment depending on their genetic potential. Here we present metagenome-assembled genomes (MAGs) for the bacterial and archaeal community in floodplain sediment samples taken June to October 2020 at two locations (OBJ1 and OBJ2) near the confluence of the Oh-Be-Joyful Creek and Slate River. The site is one of the field sites in focus for the Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory Groundwater Quality Science Focus Area (SFA) program. Sediment samples from a deep soil pit were collected from 30 cm depth below surface to just above the cobble layer (~190-250 cm depth) at discrete depths every 40 cm for microbial analyses. A total of 35 metagenomes were sequenced through the Joint Genome Institute (JGI) and can be found under Genomes Online Database (GOLD) sequencing project: Gs0142591. Metagenomes were assembled, binned, and refined using metawrap to generate MAGs (>50% complete and < 10% contamination based on checkM scores). This dataset includes a zip file of 2848 MAG fasta files and a csv file with quality, taxonomic classification (Genome Taxonomy Database Release RS220), and metagenome accessions for MAGs. This dataset also includes a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata and a data dictionary (dd.csv) file that contains column/row headers used throughout the files along with a definition, units, and data type. Part of this work was performed at SLAC Accelerator Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-76SF00515.

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